Nucleotide sequence divergence among DNA fractions of different syngens of Tetrahymena pyriformis
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چکیده
منابع مشابه
The Distribution of Dna among Dividing Mitochondria of Tetrahymena Pyriformis
A squash technique was developed for log phase Tetrahymena pyriformis which permitted the resolution of over 100 individual mitochondria from a single cell. Mitochondria incorporated thymidine at all stages of the cell cycle, even when nuclear DNA synthesis was not occurring. During the stage of macronuclear DNA synthesis, however, there was a significant increase in the extent of mitochondrial...
متن کاملReplication of macronuclear DNA in the cytoplasm of Tetrahymena pyriformis.
Previous experiments showed that a synchronous population of Tetrahymena could divide even though DNA replication was blocked during the latter half of the preceding S-period byaddition of methotrexate plus uridine (M + U). Furthermore, it was found that the DNA fraction which was in replication at the time of inhibition became localized in the cytoplasm following elimination from the nucleus a...
متن کاملComplete sequence of the mitochondrial genome of Tetrahymena pyriformis and comparison with Paramecium aurelia mitochondrial DNA.
We report the complete nucleotide sequence of the Tetrahymena pyriformis mitochondrial genome and a comparison of its gene content and organization with that of Paramecium aurelia mtDNA. T. pyriformis mtDNA is a linear molecule of 47,172 bp (78.7 % A+T) excluding telomeric sequences (identical tandem repeats of 31 bp at each end of the genome). In addition to genes encoding the previously descr...
متن کاملVincristine inhibition of DNA synthesis in Tetrahymena pyriformis.
The effect of vincristine sulfate (VCR)2 on the incorporation of isotopic precursors into protein, RNA, and DNA of the pro tozoan Tetrahymena pyriformis has been studied. DNA synthesis in logartithmically growing cells of T. pyriformi.s was almost completely inhibited by concentrations of VCR which only moderately depressed RNA and protein synthesis. Under ap propriate conditions this selective...
متن کاملPresence of two DNA polymerases in Tetrahymena pyriformis.
Two DNA polymerases were detected in Tetrahymena pyriformis, strain GL. One (enzyme I) was sensitive to N-ethylmaleimide, while the other (enzyme II) was insensitive. The molecular weight of the enzymes, as determined by glycerol gradient centrifugation analysis, were approximately 130,000 and 70,000, respectively. Optimal concentration of MgCl2 was 10mM for enzyme I and 18mM for enzyme II. KCl...
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ژورنال
عنوان ژورنال: Biochemical Genetics
سال: 1974
ISSN: 0006-2928,1573-4927
DOI: 10.1007/bf00486091